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	Provedor de dados Infoteca-e (1) Autor EMBRAPA. Centro Nacional de Pesquisa de Mandioca e Fruticultura. (1) Palavra-chave Acerola (1) Rubra. (1) Variedade. (1) Tipo do documento Fôlder / Folheto / Cartilha (INFOTECA-E) (1) Ano 2004 (1) País Brazil (1) Idioma Português (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 27 Primeira ... 1 2 ... Última PCR-based DGGE and FISH analysis of methanogens in an anaerobic closed digester tank for treating palm oil mill effluent Electron. J. Biotechnol. Tabatabaei,Meisam; Zakaria,Mohd Rafein; Rahim,Raha Abdul; Wright,André-Denis G.; Shirai,Yoshihito; Abdullah,Norhani; Sakai,Kenji; Ikeno,Shinya; Mori,Masatsugu; Kazunori,Nakamura; Sulaiman,Alawi; Hassan,Mohd Ali. 16S ribosomal RNA (rRNA)-targeted fluorescent in situ hybridization combined with polymerase chain reaction (PCR)-cloning, light microscopy using Gram stains, scanning electron microscopy and denatured gradient gel electrophoresis were used to reveal the distribution of methanogens within an anaerobic closed digester tank fed with palm oil mill effluent. For specific detection of methanogens, 16S rRNA-cloning analysis was conducted followed by restriction fragment length polymorphism (RFLP) for presumptive identification of methanogens. To cover the drawbacks of the PCR-cloning study, the organization of the microorganisms was visualized in the activated sludge sample by using fluorescent oligonucleotide probes specific to several different methanogens,... Tipo: Journal article Palavras-chave: Anaerobic digestion; DGGE; FISH; Methanogens; POME. Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300012 População microbiana em solo cultivado com soja e tratado com diferentes herbicidas em área de cerrado no estado de Roraima Acta Amazonica Zilli,Jerri Édson; Smiderle,Oscar José; Neves,Maria Cristina Prata; Rumjanek,Norma Gouvêa. Este trabalho objetivou avaliar o impacto de herbicidas à base de glyphosate, imazaquin e trifluralin na biomassa microbiana do solo, na comunidade bacteriana associada ao rizoplano de soja e também na nodulação das plantas de soja. As avaliações foram realizadas por um período de 60 dias, em dois sistemas de manejo do solo: semeadura direta na palha (SD) e semeadura convencional (SC), que receberam a aplicação dos herbicidas glyphosate e, imazaquin e trifluralin, respectivamente. Ao longo do período estudado o imazaquin, na área de SD, ocasionou redução da biomassa microbiana e, também alterou o perfil bacteriano analisado por eletroforese em gel com gradiente desnaturante (DGGE) de forma mais intensa, que o glyphosate. Na área de SC não houve efeito... Tipo: Info:eu-repo/semantics/article Palavras-chave: Biomassa Microbiana; Glyphosate; Imazaquin; Trifluralin; DGGE. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672007000200005 Population Changes of Eubacteria, Sulfate-Reducing Bacteria and Methanogenic Archaea in an Anaerobic Reactor Processing Ethanol Distillery Vinasse BABT Brown,Adis Ivonne Terry; Pozzi,Eloisa; Damianovic,Marcia Helena Rissato Zamariolli; Briones,Homero Enrique Urrutia; Ortiz,Leslie Ester Abarzúa; Pires,Eduardo Cleto. Abstract The microbiological characterization by molecular techniques (DGGE and quantitative PCR) of Archaea, Bacteria domain and sulfate-reducing bacteria (SRB) from a laboratory scale Up-flow Anaerobic Sludge Blanket (UASB) processing sugar cane vinasse was performed during the operational phase with increasing organic loads. The organic load removal efficiency was between 97% and 75% for volumetric organic loads (VOL) in the range of 0.6 to 15.4 kgCOD.m-3.d−1 and for higher VOL (until 27.0 kgCOD.m-3 .d−1) the removal efficiency decreased to 48%. Archaea represented the majority of the estimated population (107 copies of 16S RNA ribosomal gene. mL-1) followed by bacteria (106 copies of 16S RNA ribosomal gene. mL-1) and sulfate-reducing bacteria (SRB)... Tipo: Info:eu-repo/semantics/article Palavras-chave: UASB reactor; Microbial characterization; Methanogenesis; Sulfidogenesis; DGGE; Quantitative PCR. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100203 Structure and composition of bacterial and fungal community in soil under soybean monoculture in the Brazilian Cerrado BJM Bresolin,J.D; Bustamante,M.M.C; Krüger,R.H; Silva,M.R.S.S; Perez,K.S. Soybean is the most important oilseed cultivated in the world and Brazil is the second major producer. Expansion of soybean cultivation has direct and indirect impacts on natural habitats of high conservation value, such as the Brazilian savannas (Cerrado). In addition to deforestation, land conversion includes the use of fertilizers and pesticides and can lead to changes in the soil microbial communities. This study evaluated the soil bacterial and fungal communities and the microbial biomass C in a native Cerrado and in a similar no-tillage soybean monoculture area using PCR-DGGE and sequencing of bands. Compared to the native area, microbial biomass C was lower in the soybean area and cluster analysis indicated that the structure of soil microbial... Tipo: Info:eu-repo/semantics/article Palavras-chave: Savanna; Land use; Cropland; Microbial Communities; DGGE. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200021 Subseafloor archaeal communities: from the surface to a deep hot biosphere? ArchiMer Roussel, Erwan. The sub-seafloor biosphere may contain two thirds of Earth's total prokaryotic biomass. The large and active microbial populations buried in the sub-seafloor play a key role in global biogeochemical cycles. However, little is known about these prokaryotic communities. The depth limit of this sub-seafloor biosphere is still unreached, and elevated temperatures as well as insufficient energy sources are the likely factors limiting life at depth. Interestingly, archaeal communities with unknown physiologies and no cultured relatives seem to have a key role in deep marine sediments and hydrothermal ecosystems. As most deep biosphere microorganisms detected so far have been extremely resistant to cultivation, molecular approaches remain to date the most... Tipo: Text Palavras-chave: Sediment; Hydrothermal system; Ultramafic; CM DGGE; DGGE; PmoA; DsrA; McrA; AmoA; 16SrRNA; Bacteria; Archaea; Deep biosphere; Sédiment; Écosystème hydrothermal; Ultramafique; CM DGGE; DGGE; PmoA; DsrA; McrA; AmoA; 16SrRNA; Bacteria; Archaea; Biosphère profonde. Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/these-4630.pdf Tank bromeliad water: similar or distinct environments for research of bacterial bioactives? BJM Carmo,F.L.; Santos,H.F.; Peixoto,R.S.; Rosado,A.S.; Araujo,F.V.. The Atlantic Rainforest does not have a uniform physiognomy, its relief determines different environmental conditions that define the composition of its flora and fauna. Within this ecosystem, bromeliads that form tanks with their leaves hold water reservoirs throughout the year, maintaining complex food chains, based mainly on autotrophic and heterotrophic bacteria. Some works concluded that the water held by tank bromeliads concentrate the microbial diversity of their ecosystem. To investigate the bacterial diversity and the potential biotechnology of these ecosystems, tank bromeliads of the Neoregelia cruenta species from the Atlantic Rainforest in Brazil were used as models for this research. Bacteria isolated from these models were tested for... Tipo: Info:eu-repo/semantics/other Palavras-chave: Bromeliad; Atlantic Rainforest; Bacteria communities; DGGE. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100024 Use of molecular approach to verify the influence of a eutrophic lagoon in the nearby ocean's bacterioplankton communities BJM Taketani,Rodrigo Gouvêa; Araujo,Fábio Vieira; Weerelt,Margaretha Denise Maria van; Sanchez-Nunez,Maria Leonisa; Aniszewski,Erick; Rosado,Alexandre Soares. Rodrigo de Freitas lagoon is an eutrophic aquatic environment. The waters from the lagoon are released to the sea at Ipanema and Leblon beaches, through Jardim de Alah channel. In this work, the influence of these waters on the bacterial communities of these beaches was investigated. Eleven sampling stations were set between the lagoon and the beaches, and the samples were analyzed by molecular and microbiological parameters. PCR-DGGE of the DNA extracted from the samples was performed using rpoB primers. Preliminary results indicate that all used approaches could reveal the influence of the lagoon on the beaches bacterial communities. Tipo: Info:eu-repo/semantics/article Palavras-chave: DGGE; Diversity; Bacterioplankton; Eutrophic lagoon. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500017 Registros recuperados: 27 Primeira ... 1 2 ... Última

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